Abstract
A novel immunoaffinity (IA) clean-up procedure for the isolation of mycotoxins from maize extracts is described. Commonly, mycotoxins are eluted with organic solvents from IA columns after isolation. The new procedure uses water at 70 °C as alternative. The resulting eluate is suitable for direct and complete injection onto a reversed-phase liquid-chromatography column. Evaporation, reconstitution or dilution are not required. The procedure has been tested for a variety of mycotoxins, including deoxynivalenol (DON) zearalenone, T-2 and HT-2 toxins, aflatoxins and ochratoxin A in an array of different matrices. The example of DON in maize is discussed in more detail and evaluated for repeatability (5%, n=10) and recovery (95%, n=10).
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