Abstract
Aim. To assess the mechanisms of immunoadjuvant activity of marine bacteria exopolysaccharides (PS) in normal and immunosuppressive conditions in vivo. Material and methods. Experimental studies were carried out on male ВALB/c mice, immunized with compositions of PS or aluminum hydroxide and ovalbumin (OVA). Immunosuppression was induced by triple intraperitoneal administration of dexamethasone (2 mg/kg). Serum levels of specific antibodies (IgG, IgG1, and IgG2a) and cytokines (IFN-γ, IL-2, IL-10) were determined. Results. The inclusion of PS to the immune compositions led to the formation of an enhanced specific IgG, IgG1, and IgG2a to OVA, comparable to the effect of aluminum hydroxide. The studied PS also contributed to an increase in the level of both pro- and anti-inflammatory cytokines. Conclusion. PS from marine bacteria act as adjuvants in normal and immunosuppressive conditions, stimulating a mixed Th1 (IgG2a, INF-γ) and Th2 (IgG1, IL-10) immune response to OVA.
Highlights
Immunosuppression was induced by triple intraperitoneal administration of dexamethasone (2 mg/kg)
The inclusion of PS to the immune compositions led to the formation of an enhanced specific IgG, IgG1, and IgG2a to OVA, comparable to the effect of aluminum hydroxide
The studied PS contributed to an increase in the level of both pro- and anti-inflammatory cytokines
Summary
P. Somov Research Institute of Epidemiology and Microbiology, Vladivostok, Russian Federation 2 G. B. Elyakov Pacific Institute of Bioorganic Chemistry, Vladivostok, Russian Federation. Исследование механизмов иммуноадъювантного действия экзополисахаридов (ПС) морских микроорганизмов в условиях нормы и иммуносупрессии in vivo. При включении ПС в состав иммунных композиций у животных выявлено усиление специфического иммунного ответа к ОВА, сопоставимое с эффектом гидроокиси алюминия. Под влиянием исследуемых ПС также увеличивался уровень как про-, так и противовоспалительных цитокинов. ПС из морских бактерий действуют как адъюванты в условиях нормального и иммуносупрессированного организма, стимулируя смешанный Th1 (IgG2а, INF-γ) и Th2 (IgG1, IL-10) иммунный ответ к ОВА. Ключевые слова: ПС морских бактерий; адъюванты; дексаметазон; овальбумин, цитокины. Н. Иммуноадъювантная активность экзополисахаридов морских бактерий в условиях нормы и иммуносупрессии.
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