Abstract
In this study, we examined the tissue distribution of estrogen receptor alpha (ERα) and progesterone receptor (PR) in different compartments of the buffalo ovary during follicular and luteal phases of the estrous cycle. The receptors were localized by immunohistochemistry. Image analysis was done to quantify the immune reactivity. ERα was localized in various cell types of buffalo ovaries differentially during follicular and luteal phases of the estrous cycle. Immunoreactivity of ERα was detected in the primordial, primary, secondary and tertiary follicles, atretic follicles, in cells of the deep and superficial stroma, and the tunica albuginea. Specific immunostaining was observed with anti-ERα antibodies in the nuclei of follicular cells/granulosa cells and theca cells. No reaction was observed in the ovarian surface epithelium. In the growing follicle and secondary follicle, the immunoreaction for these receptors was strong. While in the tertiary follicles weak immunoreactions were recorded in the granulosa cells and theca cells. The progesterone receptors (PR) as revealed by immunohistochemistry were localized in the nuclei of different groups of ovarian cells. It was detected in the primordial, primary, secondary and tertiary follicles, atretic follicles, in cells of the deep and superficial stroma, and the tunica albuginea and surface epithelium. PR was localized in follicular cells of preantral and antral follicles, the stroma of the ovary, endothelial cells of blood vessels. PR positivity was found in one or two granulosa cells of primordial and primary follicles, with moderate immunoreaction, but no staining in oocytes. In the antral follicles, both granulosa cells, as well as theca cells, were immunostained for PR. In the obliterative atretic follicles, the invading stromal cells were highly positive for PR. Follicular cells of the primordial follicle and granulosa cells and theca cells of tertiary follicles had statistically higher percentage positive cells in the follicular phase as compared to the luteal phase. No staining was observed in the negative controls.
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