Immuno biocompatibility and anti-quorum sensing activities of chitosan-gum acacia gold nanocomposite (CS-GA-AuNC) against Pseudomonas aeruginosa drug-resistant pathogen

Abstract

In this study, an eco-friendly green route strategy was adopted for the synthesis of highly stable, biocompatible polymer-polymer- metal-based composite with high potential anti-bacterial applications. The composite was prepared by modified ionic gelation of Chitosan –gum acacia copolymer with gold nanoparticles (Cs-GA-AuNC). Structural, functional characteristics of the synthesised nanocomposite were studied by spectroscopic, electron microscopic and X-ray diffraction (XRD) studies which reveal functionalised polymers coupled with the gold nanoparticles to form electron core dense nanostructural composite material with notable nanoarchitecture. The synthesised nanocomposite was evaluated for anti-quorum sensing activity against human pathogenic bacterial strain Pseudomonas aeruginosa by measuring the expression of Las R. Nanocomposite treatment brought about drastic reduction of Las R gene expression followed by the remarkable impact on Las R gene-based virulence factors like biofilm formation, pyocyanin production, swarming motility, and total proteolytic activity. Biocompatibility of the composite was tested on Raw cells 246.7 –murine macrophage cell line/The nanocomposite was not inducing any sign of toxicity on the tested Raw cells 246.7 by exhibiting maximum cell viability, lower level of ROS generation and reduced nitride concentration which revealed the noteworthy biocompatibility of synthesised nanocomposite. This selective biological activity shown by the nanocomposite might find its application in the drug delivery for precision medicine.