Abstract
We have previously shown that antibodies to cholera toxin (CT) reacted with the major outer membrane proteins (MOMPs) from Campylobacter jejuni strains on Western blot. Further, oral immunization with CT significantly protected against challenge with C. jejuni in an adult mouse colonization model of infection. CT and the heat-labile enterotoxin (LT) of enterotoxigenic Escherichia coli are structurally and functionally related. LT and its mutants including the double-mutant LT (R192G/L211A) (dmLT), are powerful mucosal adjuvants. Unlike LT which is reactogenic, dmLT has been shown to be safe for human use. In the current study, we determined whether rabbit anti-dmLT antibodies reacted with MOMPs from C. jejuni strains and whether immunization with dmLT would afford protection against C. jejuni. On Western blot, the MOMPs from C. jejuni 48 (Penner serotype O:19), C. jejuni 75 (O:3) and C. jejuni 111 (O:1,44) were probed with rabbit antibodies to dmLT or LT-E112K (a non-toxic LT mutant), which showed a lack of reaction. Adult BALB/c mice were orally immunized with dmLT and orally challenged with C. jejuni 48 or 111. Protection from colonization with the challenge bacteria was studied by enumerating Campylobacter colonies in feces daily for 9 days. Vaccination produced robust serum and stool antibody responses to dmLT and no antibody responses to C. jejuni MOMP. Vaccinated mice showed reduced colonization and excretion of both challenge strains compared to control mice. However, the differences were not statistically significant. The protective efficacy of the dmLT vaccine varied from 9.1% to 54.5%. The lack of cross-reaction between the MOMP and dmLT suggests that protection is not mediated by cross-reacting antibodies, but may be due to activation of innate immunity. As dmLT is safe for humans, it could be incorporated into a C. jejuni vaccine to enhance its efficacy.
Highlights
Campylobacter jejuni, a foodborne pathogen, is a major cause of bacterial diarrhea worldwide [1,2,3]
We have shown that rabbit polyclonal antibodies to cholera toxin [CT] react with the major outer membrane protein (MOMP) of C. jejuni [9] and several other species of the Campylobacter genus [10]
We have previously shown that anti-CT antibodies react with MOMPs from various strains of C. jejuni [9]
Summary
Campylobacter jejuni, a foodborne pathogen, is a major cause of bacterial diarrhea worldwide [1,2,3]. C. jejuni infection results in serious complications such as the Guillain-Barre syndrome, Reiter’s syndrome, reactive arthritis, and irritable bowel syndrome [4]. The economic burden of diarrhea and the complications due to C. jejuni are considerable [5]. Many agencies including the World Health Organization have declared the development of a vaccine against C. jejuni a priority [6, 7]. We have shown that rabbit polyclonal antibodies to cholera toxin [CT] react with the major outer membrane protein (MOMP) of C. jejuni [9] and several other species of the Campylobacter genus [10]. Oral immunization of adult mice with CT afforded significant protection against intestinal colonization with C. jejuni upon oral challenge [11]
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