Abstract
Immunization of mice with heavily irradiated syngeneic Moloney lymphoma cells evokes antibodies against the major viral envelope antigen, gp71, and the Moloney virus-induced cell surface antigen (MCSA). A9HT cells, an L-cell subline, react with the antibodies against the viral envelope antigen only; this reaction can be completely inhibited by virus or purified gp71. Reactivity to Moloney lymphoma cells (YAC) was only partially inhibited (maximum 30%) or not at all. This can be attributed to the reaction of the YAC cells with antibodies directed against MCSA, a nonvirion cell surface component according to both biological and biochemical evidence. Antibody-induced capping of gp71 or p15(E) did not change the membrane distribution of MCSA or H-2, indicating that these antigens represent distinct entities on the cell surface. MCSA showed only minimal capping and thereby differed in behavior from both H-2 and virion antigens. gp71 could be capped by the mouse antiserum as revealed by subsequent staining with monospecific anti-gp71 antiserum. Under ordinary test conditions this reactivity is overshadowed by the reaction against MCSA. The lack of MCSA capping reflects a difference in anchorage of this antigen.
Highlights
Further evidence was obtained to confirm that the mouse anti-ML serum reacted with viral envelope components on A9HT cells when we found that purified FVgp71 inhibits cytotoxicity completely (Fig. 2)
Immunization of mice with heavily irradiated syngeneic Moloney lymphoma cells evokes antibodies against the major viral envelope antigen, gp71, and the Moloney virus-induced cell surface antigen (MCSA)
A9HT cells, an L-cell subline, react with the antibodies against the viral envelope antigen only; this reaction can be completely inhibited by virus or purified gp71
Summary
The YAC and YA7C lymphomas were induced by inoculating Moloney leukemia virus (MLV) to newborn A and (A × C57BI)FI mice, respectively[9].The characteristicsof Y A C cellsurface antigens and virus production have been described in a seriesof publications [6, 10, 11]. Both tumors were maintained in the ascitesform in syngeneic mice. AgHT, a highly malignant A9 variant [12].Like other L-cellsublines, A 9 H T produces a C-type virus that shows a certain resemblance to endogenous mouse viruses. The protein content was 3.93 mg/ml [17]
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