Immunization of mice with DNA encoding fragment C of tetanus toxin

Abstract

Immunization of mice with Fragment C protein, the non-toxic C-terminal domain of tetanus toxin, will protect mice against lethal challenge with tetanus toxin. A plasmid, pcDNA3/tetC, which encodes a synthetic tetC gene expressed under the control of the human cytomegalovirus major intermediate early promoterlenhancer region, was constructed. Fragment C expression was observed in Chinese hamster ovary cells following transfection with pcDNA3/tetC. The immune response induced by intramuscular immunization with pure pcDNA3/tetC DNA was evaluated in a murine model. Anti-Fragment C serum immunoglobulin and proliferative responses in splenocytes were observed following two immunizations with pcDNA3/tetC. The major IgG subclass that recognized Fragment C was IgG2a and the stimulated splenocytes secreted high levels of interferon-γ. Sufficient anti-Fragment C serum immunoglobulins were induced by DNA-mediated immunization to protect mice against lethal challenge with tetanus toxin.