Abstract
A previous study identified that isoform 2 (Hcftt-2) of the 14-3-3 protein of Haemonchus contortus (H. contortus) could suppress immune functions of goat peripheral blood mononuclear cells (PBMCs) and might be a potential vaccine target, as neutralization of the protein function may enhance anti-parasite immunity. In this research, the recombinant Hcftt-2 was evaluated for its immunoprotective efficacy against H. contortus infection in goats. Five experimental goats were immunized twice with rHcftt-2 along with Freund’s adjuvant. The five immunized goats and five nonimmunized goats (adjuvant only) were challenged with 5000 L3-stage H. contortus larvae after 14 days of second immunization. Five nonimmunized and uninfected goats (adjuvant only) were set as the uninfected group. A significant increase in the serum immunoglobin G(IgG) and serum IgA levels were identified in the rHcftt-2 immunized animals. The mean eggs per gram in feces (EPG) and the worm burdens of rHcftt-2 immunized group were reduced by 26.46% (p < 0.05) and 32.33%, respectively. In brief, immunization of goats with rHcftt-2 induced moderate protection against H. contortus challenge.
Highlights
Helminth parasites are a worldwide problem, resulting in economic losses in the livestock industry.Haemonchus contortus is a highly pathogenic blood-sucking gastrointestinal nematode of the abomasum of small ruminants
Many native and recombinant proteins have been investigated in different immunization trials against H. contortus and most cases demonstrated variable reduction in fecal egg output and worm burdens [3,6,7,8,9]
Sera from infected goats revealed the presence of sero-reactive bandsthe at molecular weight of 48 kDa
Summary
Helminth parasites are a worldwide problem, resulting in economic losses in the livestock industry. Haemonchus contortus is a highly pathogenic blood-sucking gastrointestinal nematode of the abomasum of small ruminants. Infections with this nematode can cause anemia, weight loss, and even death [1,2,3,4]. Current methods of prevention and control depend on the use of anthelmintics [3]. The appearance of anthelmintic-resistant strains, the environmental pollution, and the toxic residues in animal products has led to the urgent need for the development of more effective vaccines [5]. DNA vaccines have been tested and showed partial protection against
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