Immune-modulatory properties of cinnamaldehyde are associated with the induction of apoptosis in immune cells

Abstract

Event Abstract Back to Event Immune-modulatory properties of cinnamaldehyde are associated with the induction of apoptosis in immune cells Anna Moskovskich1, Cristina Gomez-Casado2, Anna Willensdorfer1, Josef Singer3, Heidemarie Fuchs4, Franziska Roth-Walter1 and Erika Jensen-Jarolim1, 3* 1 Messerli Research Institute, Comparative Medicine; University of Veterinary Medicine Vienna, Medical University of Vienna, University of Vienna, Austria 2 Technical University Madrid, Center for Plant Biotechnology and Genomic, Spain 3 Medical University of Vienna, Department of Pathophysiology and Allergy Research, Austria 4 University of Applied Sciences Technikum Wien, Department of Biochemical Engineering, Austria Background: Cinnamaldehyde (CA) has been reported to have anti-inflammatory, anti-bacterial, anti-fungal, anti-mutagenic, chemoprotective and anticarcinogenic activity. Here, we further investigated the immune-modulating capacity of CA. Methods: NF-κB activation by CA (0-10µg/ml) alone or in combination with lipopolysaccharide (LPS) was assessed using the THP1XBlue© human monocytic cell line transfected with an alkaline phosphatase reporter. IL10 and nitric oxide (NO) secretion in murine RAW264.7 cells and human peripheral blood mononuclear cells (PBMCs) stimulated with CA alone or in conjunction with LPS were analyzed. Human PBMCS were stimulated with different concentrations of CA alone or in combination with LPS for 24h and subsequently stained with AnnexinV-FITC and propidium iodide to assess cell death and apoptosis. Results: Incubation with CA resulted in a concentration-dependent inhibition to a maximum of 50% of NFκB in LPS-stimulated THP1-cells. Similarly, NO as well as IL10 secretion induced by LPS in RAW264.7 cells were blocked by addition of CA. Induction of apoptosis in human PBMCS was triggered by CA alone or in conjunction with LPS. These results correlated with a dose-dependent decrease in IL10 by addition of CA in LPS-stimulated PBMCS. Conclusion: Anti-inflammatory properties of CA are likely due to blocking of NFκB-pathway and induction of apoptosis in immune cells. Keywords: Cinnamaldehyde, Apoptosis, NF-kappa B, Lymphocytes, Immunosuppressive Agents Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Translational immunology and immune intervention Citation: Moskovskich A, Gomez-Casado C, Willensdorfer A, Singer J, Fuchs H, Roth-Walter F and Jensen-Jarolim E (2013). Immune-modulatory properties of cinnamaldehyde are associated with the induction of apoptosis in immune cells. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00968 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 26 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Prof. Erika Jensen-Jarolim, Messerli Research Institute, Comparative Medicine; University of Veterinary Medicine Vienna, Medical University of Vienna, University of Vienna, Vienna, Austria, erika.jensen-jarolim@meduniwien.ac.at Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Anna Moskovskich Cristina Gomez-Casado Anna Willensdorfer Josef Singer Heidemarie Fuchs Franziska Roth-Walter Erika Jensen-Jarolim Google Anna Moskovskich Cristina Gomez-Casado Anna Willensdorfer Josef Singer Heidemarie Fuchs Franziska Roth-Walter Erika Jensen-Jarolim Google Scholar Anna Moskovskich Cristina Gomez-Casado Anna Willensdorfer Josef Singer Heidemarie Fuchs Franziska Roth-Walter Erika Jensen-Jarolim PubMed Anna Moskovskich Cristina Gomez-Casado Anna Willensdorfer Josef Singer Heidemarie Fuchs Franziska Roth-Walter Erika Jensen-Jarolim Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.