Abstract
BackgroundMycoplasma hyopneumoniae (Mhp) and porcine circovirus type 2 (PCV2) are two important pathogens causing Mycoplasma pneumonia of swine (MPS) and porcine circovirus diseases and porcine circovirus-associated diseases (PCVDs/PCVADs), respectively, and resulted in considerable economic loss to the swine industry worldwide. Currently, vaccination is one of the main measures to control these two diseases; however, there are few combination vaccines that can prevent these two diseases. To determine the effect of combination immunization, we developed capsid-derived (Cap) virus-like particles (VLPs) of PCV2 and a new recombinant chimera composed of the P97R1, P46, and P42 antigens of Mhp. Then we investigated the immune responses induced by the immunization with this combination vaccine in mice and piglets.ResultsThe high level antibodies against three protein antigens (P97R1, P46, and P42 of Mhp) were produced after immunization, up to or higher than 1:400,000; the antibody levels in Pro group continuously increased throughout the 42 days for all the antigens tested. The lymphocyte proliferative response in PCV2 group was stronger than that in PBS, VP, Mhp CV in mice. The antibody levels for Cap remained stable and reached the peak at 35 DAI. The IFN-γ and IL-4 in sera were significantly enhanced in the Pro group than that in the negative control-VP group on Day 14 and 28 post-the first immunization in piglets.ConclusionsAbove all, the combination immunization could induce humoral and cellular immune responses against all four antigens in mice and piglets. Therefore, our approach is a simple and effective vaccination strategy to protect pigs against MPS and PCVD/PCVAD.
Highlights
Mycoplasma hyopneumoniae (Mhp) and porcine circovirus type 2 (PCV2) are two important pathogens causing Mycoplasma pneumonia of swine (MPS) and porcine circovirus diseases and porcine circovirusassociated diseases (PCVDs/porcine circovirus diseases and porcine circovirus-associated diseases (PCVADs)), respectively, and resulted in considerable economic loss to the swine industry worldwide
The purity of the pET32a vector, rP97R1, rP46, rP42, rP97R1P46P42, and capsid (Cap) proteins were analyzed by SDS-PAGE (Fig. 2) and Western blotting (Fig. 3, the full-length blots are presented in Supplementary Fig. (1, 2, 3, 4 and 5) for A to E respectively, the cropped area of the picture was marked with a red box line)
It demonstrated that the expression and purification of recombinant proteins were in line with our expectations, and these values corresponded to the expected molecular weights of pET32a vector protein (20.4 kDa), rP97R1 (31.3 kDa), rP46 (28.9 kDa), rP42 (37.4 kDa), rP97R1P46P42 (61.2 kDa), and Cap (38.8 kDa)
Summary
Mycoplasma hyopneumoniae (Mhp) and porcine circovirus type 2 (PCV2) are two important pathogens causing Mycoplasma pneumonia of swine (MPS) and porcine circovirus diseases and porcine circovirusassociated diseases (PCVDs/PCVADs), respectively, and resulted in considerable economic loss to the swine industry worldwide. Mycoplasma pneumonia of swine (MPS), known as porcine enzootic pneumonia (PEP) or enzootic pneumonia (EP), is mainly caused by the pathogen Mycoplasma hyopneumoniae (Mhp). This chronic respiratory disease is widely spread all over the world and causes considerable economic loss [1, 2]. Many researchers have focused on studying this conserved region The molecular chaperone DnaK (P42) and membrane surface protein (P46) are another two important adhesion factors, which can be potentially used in vaccine research [4, 5, 12,13,14,15]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
