Abstract
Trichinella spiralis is a parasitic helminth that can infect almost all mammals, including humans. Trichinella spiralis infection elicits a typical type 2 immune responses, while suppresses type 1 immune responses, which is in favour of their parasitism. DNA vaccines have been shown to be capable of eliciting balanced CD4+ and CD8+ T cell responses as well as humoral immune responses in small-animal models, which will be advantage to induce protective immune response against helminth infection. In this study, serine protease (Ts-NBLsp) was encoded by a cDNA fragment of new-born T. spiralis larvae, and was inserted after CMV promoter to construct a DNA vaccine [pcDNA3·1(+)-Ts-NBLsp]. Ts-NBLsp expression was demonstrated by immunofluorescence. Sera samples were obtained from vaccinated mice, and they showed strong anti-Ts-NBLsp-specific IgG response. Mice immunized with the pcDNA3·1(+)-Ts-NBLsp DNA vaccine showed a 77·93% reduction in muscle larvae (ML) following challenge with T. spiralis ML. Our results demonstrate that the vaccination with pcDNA3·1(+)-Ts-NBLsp plasmid promoted the balance of type 1 and 2 immune responses and produced a significant protection against T. spiralis infection in mice.
Highlights
Trichinella spp. are intestinal nematode parasites that can cause trichinellosis in humans and animals (Dupouy-Camet, 2000)
DNA sequence analysis indicated that the amplified fragment of TsNBLsp gene consisting of 1209 bp was correctly cloned into prokaryotic expression vector pet28a and eukaryotic expression vector pcDNA3·1(+)
Western blot results demonstrated that the expression of recombinant protein rTs-NBLsp could be induced after addition of IPTG
Summary
Trichinella spp. are intestinal nematode parasites that can cause trichinellosis in humans and animals (Dupouy-Camet, 2000). Infection occurs when humans consume raw or undercooked meat of different animal origins containing T. spiralis muscle larvae (ML). Trichinellosis is a public health hazard, and it an economic problem in animal production and food safety (Dorny et al 2009). It is difficult to control this zoonosis due to its wide distribution of domestic and wild animal reservoirs (Wang and Cui, 2001; Wang et al 2006; Cui et al 2011; Murrell and Pozio, 2011). Useful and stable anti-Trichinella vaccines that can be used in animal husbandry have not been developed yet. It is necessary to develop a vaccine to prevent Trichinella infection in domestic animals and humans
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