Abstract
The development of cellular immunity to a syngeneic squamous cell carcinoma in Wistar rats was studied by in vitro microcytotoxicity assay. Reactivity of lymphocytes from lymph nodes, spleen and blood was tested throughout the period of tumour growth. Maximum lymphocyte cytotoxicity against the tumour was observed at 2 weeks in regional lymph nodes, 4 weeks in intermediate nodes, spleen and blood, and 6 weeks in distant nodes; the intensity of these cytotoxic responses subsequently declined. In the regional nodes, lymphocytes became totally unresponsive despite the maintenance of significant cytotoxicity in intermediate nodes, spleen and blood. Local anergy may account for tumour spread to the regional node in an otherwise immunocompetent host. This anergy may be due to high local concentration of tumour antigen or antigen-antibody complexes, but it was not associated with selective changes in T and B cell proportions.
Highlights
Summary.-The development of cellular immunity to a syngeneic squamous cell carcinoma in Wistar rats was studied by in vitro microcytotoxicity assay
We have observed a similar loss of lymphocyte reactivity in rats bearing a syngeneic squamous cell carcinoma during a study of the development of cellular immunity in various lymphoid tissues
We have observed the development of local lymphocyte anergy in animals with progressively growing tumour
Summary
Inbred Wistar rats obtained from Professor R. Tumour cell suspensions used for inoculation and for cytotoxicity assays were taken from a common stock of frozen cells preserved w%ith. After 4 hours at 37°C, the Previous experiments have shown fresh and plates were gently washed twice with warm frozen tumour cells to be effective as medium to remove non-adhering lymphocytes antigenic targets in the microcytotoxicity and incubated for a further 40-48 hours at assay. Rats were inoculated subcutaneously in the medial aspect of the right thigh with 103 viable tumour cells, previously shown to produce tumours in all animals and death in 8-10 weeks. Cytotoxicity was expressed as the percentage reduction in the mean number of surviving tumour cells in test (Nt) versus control (Nc) wells, i.e. 2, 4, 6 and 8 weeks after tumour inoculation, the volumes of the primary tumours detercytotoxicity_=_-Nc Nc-Nt- x 100. Student's t-tests were performed to estimate the statistical significance of differences between cytotoxicity means and a difference was considered significant at the P < 0-05 level
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