Immune response related to Pelibuey sheep naturally infected with gastrointestinal nematodes in a tropical region of Mexico

Abstract

We analysed the immune response involved in sheep naturally infected with gastrointestinal (GI) nematodes. Fifteen Pelibuey lambs were grazed in paddocks contaminated with GI nematodes for 13weeks. To assess the infection, the number of eggs per gram (epg) and the percentage of packed cell volume (pcv) were evaluated. Blood and abomasal tissue samples were collected at week 8 post-infection to analyse the expression levels of IL-4, IL-5, IL-6, IL-8, IL-13, TGF-β and FCεR1A genes. The nematode Haemonchus contortus was the main species identified. In addition, two groups of lambs were classified based on the x±SE of epg and pcv values: G-1, with 151 ± 28 and 29±0.33%, respectively, and G-2, with 475±59.5 and 26±0.38%, respectively. For G-1, upregulation of IL-4, IL-8, IL-13, TGF-β and FCεR1A genes from 2.42- to 14.99-fold was observed in blood and abomasal tissue samples (p>.05), and IL-5, IL-8 and TGF-β genes had significant gene expression levels in blood (p<.05). For G-2, moderate gene expression levels, ranging from 1.22- to 3.45-fold, were observed in abomasal tissue (p>.05), and the IL-5 gene presented significant gene expression in blood (p<.05). Strong positively correlated values (r) between pcv and IL-4, IL-8 and TGF-β genes were observed in G-1. In contrast, significant negative correlations between epg and IL-4, IL-5 and FCεR1A genes indicate acute infection for G-2. Our results suggest that IL-4, IL-5, IL-13, TGF-β and FCεR1A genes are important modulators of GI nematode infections of Pelibuey lambs.