Immune response in animals with experimental Rauscher leukemia to mononuclear stem cells and Subalin

Abstract

The results of the effect of immunomodulating biologically active drugs on the immune response of laboratory animals are presented. To study the processes, a model of experimental Rauscher leukemia was used in mice of pure lines. The morphological changes in the peripheral blood of Bagg Albino C (BALB / c) mice with experimental Rauscher's leukemia after the use of mononuclear stem cell and Subalin preparations were studied. Groups of animals were formed for the experiment: control and three experimental (infected). The animals of the experimental groups were examined simultaneously by intraperitoneal injection of the appropriate biomaterial. It was found that the use of mononuclear stem cells and Subalin does not prolong the life of infected animals. During the observation period, all infected mice died within 11 months. After infection with Rauscher leukemia, a quantitative and qualitative change in blood cells occurs. A shift to the left was noted in the leukogram, eosinophilia and monocytosis were revealed. In the process of experimental reproduction of the chronic form of the disease, the size of the spleen of mice was estimated. An increase in the spleen was observed in the experimental group of animals infected with the Rauscher leukemia virus on the 4th week after infection. It was found that Subalin has a pronounced effect on the leveling of hematological parameters and leukopoiesis in infected mice. In the group where stem cells were used together with the antigen, a slight decrease in the number of lymphocytes and an increase in stab and segmented neutrophils were recorded. In the experimental groups with the use of mononuclear stem cells and Subalin, an increase in young cells in the leukogram was noted.