Abstract
e22200 Background: Current evidence suggests the presence of cancer stem cells (CSC) in epithelial ovarian cancer (EOC) and their role in chemoresistance and relapse. CD44+/CD24-, CD117+ and CD133+ expression have been reported as potential markers for CSC. Methods: Tumor cells from 5 patients with chemo-naive EOC and cells of ovarian and tubal epithelium from 5 healthy menopausal women undergoing surgery for benign pathology were cultured in standard medium (mediumA) and medium for embrionic cells (mediumB).Cells forming non-adherent spheres where considered CSC-like, and adherent cells as non-CSC-like. The percentages of CD24+, CD44+, CD117+, CD133+ and VEGF-R+ cells were determined by flow-cytometry. Results: After 1-7 week sphere formation was observed in all EOC-cells cultured in mediumB but in none of the healthy controls. Mean percentages of markers expression are shown below. Conclusions: Contrary to published data, expression of CD44+/CD24- was lower in CSClike; in Non-CSClike CD133+ was higher in EOC-cells compared to controls. Inmunophenotype of CSClike is still lacking and further investigation is warranted. [Table: see text] [Table: see text]
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