Immune functionalization of outer membrane proteins (OMPs) for enteropathogenic E.coli in vivo

Abstract

This study aimed to reveal the susceptibility of Outer Membrane Proteins (OMPs) to Enteropathogenic E.coli (EPEC) after linking it to an immunosuppressant (from natural sources)to stimulate the immune system of laboratory animals as a primitive attempt to initiate the manufacture of a vaccine against the above bacteria and reduce of the fatalities that occur in children under the age of five when injuredRelying on some common methods, outer membrane proteins (OMPs) were separated from EPEC bacteria membrane in an amount of 200 mg / 20 L of culture medium.The molecular weight and purity of this protein were detected using Sodium Dodecyl Sulfate-Polyacrylamide gel ( SDS-PAGE), as the outer membrane proteins OMP appeared in the form of a ladder containing successive bundles consisting of (9-13) bundles of polypeptides, whose molecular weight ranges between (15-125) kDa.The ability of EPEC bacteria, its outer membrane proteins (OMPs),the immune helper (olive oil), and the foreign protein complex with the immune helper to induce systemic immunity through changes in the antibody titer averages were studied.A significant increase in the titer of antibodies was observed in the treated mice (IgG and IgA) compared to the positive control group and those that appeared in the animals before treatment.