Abstract
This study was carried out to assess the protective immune effect of mixed culture of Lactobacillus acidophilus and Lactobacillus gasseri in mice infected with Salmonella typhimurium. Parameters of evaluation were total and absolute count of leukocyte and phagocytosis. Fifteen albino mice divided into five groups and designated as follows: CG used as negative control, SG was infected with 0.1 ml Salmonella typhimurium 2.5×107 cfu/ml and used as a positive control, AC was treated with 0.1ml Lactobacillus acidophilus 1×109 cfu/ml and infected with Salmonella typhimurium 2.5×107 cfu/ml, GG was doused with Lactobacillus gasseri and infected with Salmonella typhimurium, AG was fed with 0.1 ml mixed culture of Lactobacillus acidophilus and Lactobacillus gasseri 1×109 cfu/ml and infected with Salmonella typhimurium 2.5×107 cfu/ml. Results indicated that mice treated with viable Lb.acidophilus and Lb.gasseri showed a significant protective immune effect compared with positive and negative control, while mice fed with mixed culture of Lb.acidophilus and Lb.gasseri exhibited less protective effect against Salmonella typhimurium compared with groups fed with monoculture of Lactobacillus.
Highlights
Probiotics are nonpathogenic organisms in foods that can exert a positive influence on the host’s health [1]
Results showed that mice fed with monoculture of Lactobacillus acidophilus and Lactobacillus gasseri exhibited a statistically increase in total and absolute count of leukocyte and phagocytic index compared with positive and negative control
Results in this study demonstrated that mice fed with mixed culture of Lb. acidophilus and Lb. gasseri caused a statistically decrease in total and absolute count of leukocyte compared with negative control but this result was significantly higher when compared with positive control
Summary
Probiotics are nonpathogenic organisms (yeast or bacteria, especially lactic acid bacteria) in foods that can exert a positive influence on the host’s health [1]. An essential step in the pathogenesis of typhoid fevers in mice or humans is the establishment of a systemic infection after oral challenge. Mixed Lactobacillusculture The Lactobacillus was grown in de Mann, Rogosa and Sharp (MRS) broth (Merck) media for 18 hr at a 370c This activated culture was centrifuged at 2000 gat 4oC and resuspended in phosphate-buffered saline with pH 7, in order to obtain 109colony forming units (cfu)/ml which were obtained by using McFarland's method. GroupAG: This group was fed with 0.1ml of 109cfu/ml Lactobacillus acidophilus culture, and infected with 0.1ml of 2.5×107 cfu/ml culture of Salmonella typhimurium. GroupAG: This group was fed with 0.1ml of 109cfu/ml of mixed Lactobacillus acidophilus and Lactobacillus gasseri cultures, and doused with 0.1ml of 2.5 × 107 cfu/ml culture of Salmonella typhimurium. After 6th day infection with Salmonella, mice were sacrificed by cervical dislocation and the blood samples of each mouse were collected to evaluate immunological parameters [3]
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