Abstract
BackgroundThe Gram-negative pathogen Bordetella bronchiseptica causes acute and chronic respiratory infection in a variety of animals. Currently, there is no vaccine to prevent these infections. To identify useful candidate antigens for such a vaccine, five B. bronchiseptica genes including amino acid ATP-binding cassette transporter substrate-binding protein (ABC), lipoprotein (PL), outer membrane porin protein (PPP), leu/ile/val-binding protein (BPP), and conserved hypothetical protein (CHP) were cloned and the recombinant proteins were expressed. The immune responses of mice to vaccination with individual recombinant proteins were measured.ResultsEach of the tested recombinant proteins induced a high antibody titer. PPP and PL showed protective indices against challenges with B. bronchiseptica. The protection ratios were 62.5 and 50 %, respectively, compared with 12.5 % for control vaccinations. The protection ratios of ABC, BPP, and CHP were not significantly different from the controls. IgG-subtype and cytokine analysis demonstrated that PPP and PL can induce two immune responses: a humoral immune response and a cell-mediated immune response. The humoral immunity-mediated, Th2-type response dominated.ConclusionThe identification of PPP and PL, which offer immune-protective potential, identifies them as candidates for the development of a diagnostic test or a vaccine for B. bronchiseptica.
Highlights
The Gram-negative pathogen Bordetella bronchiseptica causes acute and chronic respiratory infection in a variety of animals
B. bronchiseptica infections are often endemic in commercial rabbitries, where they and difficult to control due to the rapid spread and persistence of the infection [2]
Sequence analysis showed that the homology between the amplified genes and the corresponding reference sequences reached more than 97 %, which was consistent with the deduced amino acid sequences
Summary
The Gram-negative pathogen Bordetella bronchiseptica causes acute and chronic respiratory infection in a variety of animals. To identify useful candidate antigens for such a vaccine, five B. bronchiseptica genes including amino acid ATP-binding cassette transporter substrate-binding protein (ABC), lipoprotein (PL), outer membrane porin protein (PPP), leu/ile/val-binding protein (BPP), and conserved hypothetical protein (CHP) were cloned and the recombinant proteins were expressed. The immune responses of mice to vaccination with individual recombinant proteins were measured. Bordetella bronchiseptica is an important bacterial pathogen that causes a number of respiratory diseases in livestock and poultry. The development of a new vaccine is critical to the prevention and control of B. bronchiseptica infection. We tested the recombinant proteins for immunogenicity and protection against B. bronchiseptica in mice to find novel immune-protective antigens
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