Immune complexes in blood serum of calves with clinical symptoms of bronchopneumonia

Ivana Jaglicic,Olivera Savic,Natalija Fratric,Dragan Gvozdic,Vesna Ilic

doi:10.2298/vetgl1102013f

Abstract

Pneumonia in preruminant calves is a multifactorial disease. Infectious agents, the environment, management and the immune status of the calves are all important factors in determining the outcome of an infection. Until today, the level and composition of circulating immune complexes in preruminant calves with pneumonia have not been studied in detail. We performed this work with the aim to determine whether pneumonia in three-month-old calves is followed by changes in the immune complex level and changes in the ?-globulin level as their possible constituents. Immune complexes from the calves? sera were isolated by polyethylene glycol (PEG) precipitation methods. Optical density at 350 nm (OD350) of redissolved precipitates was measured to determine the circulating immune complexes level. The OD350 level of PEG precipitates of calves with pneumonia at the time of diagnosis was 0.577?0.206 and it was statistically significantly higher (p<0.001) than OD350 the level of PEG precipitates of healthy calves (0.286?0.080). Electrophoretic analysis of sera and PEG precipitates showed that both slow and fast ?-globulins are found among serum and immune-complexes' ?-globulins, but the concentration of fast ?-globulins was significantly lower in sera of diseased calves. The level of PEG precipitable immune complexes was not correlated with the concentration of serum and PEG precipitable g-globulins. The results of this study have shown that by relatively simple PEG precipitation assay it is possible to detect an increased level of circulating immune complexes in calves with pneumonia. This can be used as an additional diagnostic parameter for the detection and follow up of the disease.

Highlights

Vet. glasnik 65 (1-2) 13 - 24 (2011) Natalija Fratri} i sar.: Imunski kompleksi u krvnom serumu teladi sa klini~kim simptomima bronhopneumonije sa, a rezultati dobijeni ovim relativno jednostavnim testom mogu se koristiti kao dodatni parametar za dijagnozu i pra}enje toka bolesti
Ova oboljenja su uzrok velikih ekonomskih gubitaka na govedarskim farmama (Ames i sar., 1997; Snowder i sar., 2006), jer smrtnost moe da dostigne nivo i od 25% i zajedno sa cenom tretmana obolelihivotinja pove}ava tro{kove proizvodnje za vi{e od 8%
Na osnovu dobijenih rezultata moe se zaklju~iti da se pove}anje nivoa imunskih kompleksa u serumima teladi uzrasta od tri meseca obolelih od bolesti respiratornog trakta moe detektovati relativno jednostravnim PEG precipitacijskim testom, a dobijeni rezultati se mogu koristiti kao dodatni dijagnosti~ki parametar

Summary

Introduction

Vet. glasnik 65 (1-2) 13 - 24 (2011) Natalija Fratri} i sar.: Imunski kompleksi u krvnom serumu teladi sa klini~kim simptomima bronhopneumonije sa, a rezultati dobijeni ovim relativno jednostavnim testom mogu se koristiti kao dodatni parametar za dijagnozu i pra}enje toka bolesti. Cilj ovog istraivanja bio je da se utvrdi nivo i karakteristike imunskih kompleksa u serumima teladi u uzrastu od tri meseca sa oboljenjem respiratornog trakta u cilju dobijanja dodatnog dijagnosti~kog kriterijuma kod ove bolesti.

Results

Conclusion