Abstract
The objective of this study was to characterize the pulp immune cell profile in the teeth of rats treated with Zoledronic Acid (ZA). For this Male Wistar rats (n=6) received 4 intravenous infusions of ZA (0.04, 0.20 or 1.00 mg/kg) or saline. In the 70th day, they were euthanized. The first right molar was microscopically examined (H&E, toluidine blue and immunohistochemistry for CD68, Tumor Necrosis Factor (TNF)-α, Interleukin(IL)-1β, Inducible Nitric Oxide Synthase (iNOS), Nuclear Factor kappa B (NFkB) and IL-18 binding protein (IL-18bp). Were analyzed the ectasic/dilated vessels, the inflammatory cells (mast cells and mononuclear CD68 positive cells) and intensity of immunostaining for inflammatory markers in odontoblasts and non-odontoblasts pulp cells. Were used the Kruskall-Wallis/Dunn's and the chi-square tests (P<.05). There is no differences in number of animals exhibiting dilated/ectasia blood vessels (P=.242), inflammatory cells (P=.489) and in the number of mast cells (P=1.000). However, there was an augment of CD68 positive cells (P=.026), immunostaining of TNF-α (P=.020), IL-1β (P=.027), iNOS (P=.001) in odontoblasts and IL-1β (P=.027) in non-odontoblasts pulp cells dose-dependently. NFkB (nucleus and cytoplasm) and IL-18bp did not differ between groups. It was showed that ZA modified the immune cell profile in the dental pulp independent of NFkB.
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