Abstract
BackgroundCD8+ suppressor T cells exert antigen-specific suppression of the expression of hypersensitivity by activated T cells. Therefore, CD8+ suppressor T cells serve a major regulatory role for the control of active immunity. Accordingly, the number and/or activity of CD8+ suppressor T cells should be influenced by an immune response to the antigen. To test this hypothesis we used an adoptive transfer assay that measures the suppression of the expression of delayed-type hypersensitivity (DTH) by CD8+ suppressor T cells to quantify the antigen-specific suppression of DTH by these suppressor T cells.MethodsSuppressor T cells were induced in the spleens of mice by the injection of antigen into the anterior chamber of an eye. Following this injection, the mice were immunized by the same antigen injected into the anterior chamber. Spleen cells recovered from these mice (AC-SPL cells) were titrated in an adoptive transfer assay to determine the number of AC-SPL cells required to effect a 50% reduction of antigen-induced swelling (Sw50) in the footpad of immunized mice challenged by antigen.ResultsSuppression of the expression of DTH is proportional to the number of AC-SPL cells injected into the site challenged by antigen. The number of AC-SPL cells required for a 50% reduction in DTH-induced swelling is reduced by injecting a cell population enriched for CD8+ AC-SPL cells. Immunizing the mice receiving intracameral antigen to the same antigen decreases the RSw50 of AC-SPL cells required to inhibit the expression of DTH.ConclusionsThe results provide the first quantitative demonstration that the numbers of antigen-specific splenic CD8+ suppressor T cells are specifically amplified by antigen during an immune response.
Highlights
CD8+ suppressor T cells execute a Qa-1-restricted, antigenspecific suppression of activated T cells [1,2,3,4,5,6,7,8,9,10,11,12]
The results show for the first time that immunization amplifies quantitatively splenic CD8+ suppressor T cells induced by the intracameral injection of antigen
Two hundred fifty-25, 000 total spleen cells recovered from the donors that received an intracameral injection of antigen (AC-SPL cells ) were injected into the footpads of the TNP-bovine serum albumin (BSA)-immunized mice immediately after the footpad was challenged epicutaneously with Picryl chloride (PCl)
Summary
CD8+ suppressor T cells execute a Qa-1-restricted, antigenspecific suppression of activated T cells [1,2,3,4,5,6,7,8,9,10,11,12]. Immunization should induce a specific increase in CD8+ suppressor T cells responding to the antigen and/or a variable region epitope of the receptor of T cells specific for the antigen [1,2,8]. CD8+ suppressor T cells exert antigen-specific suppression of the expression of hypersensitivity by activated T cells. The number and/or activity of CD8+ suppressor T cells should be influenced by an immune response to the antigen. To test this hypothesis we used an adoptive transfer assay that measures the suppression of the expression of delayed-type hypersensitivity (DTH) by CD8+ suppressor T cells to quantify the antigen-specific suppression of DTH by these suppressor T cells
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