Immune adjuvant effect of V. cholerae O1 derived Proteoliposome coadministered by intranasal route with Vi polysaccharide from Salmonella Typhi.

Reinaldo Acevedo,Luis García,Adriana Callicó,Caridad Zayas,Oliver Pérez,Valerie A Ferro,Yisabel Aranguren,Yolanda Valdés,José Luis Pérez

doi:10.1186/1471-2172-14-s1-s10

Reinaldo Acevedo, Luis García + Show 7 more

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https://doi.org/10.1186/1471-2172-14-s1-s10

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Journal: BMC immunology	Publication Date: Feb 1, 2013
Citations: 19	License type: CC BY 2.0

Affiliation: Finlay Institute, University of Strathclyde

Abstract

The proteoliposome derived from Vibrio cholerae O1 (PLc) is a nanoscaled structure obtained by a detergent extraction process. Intranasal (i.n) administration of PLc was immunogenic at mucosal and systemic level vs. V. cholerae; however the adjuvant potential of this structure for non-cholera antigens has not been proven yet. The aim of this work was to evaluate the effect of coadministering PLc with the Vi polysaccharide antigen (Poli Vi) of S. Typhi by the i.n route. The results showed that Poli Vi coadministered with PLc (PLc+Poli Vi) induce a higher IgA response in saliva (p<0.01) and faeces (p<0.01) than Poli Vi administered alone. Likewise, the IgG response in sera was higher in animals immunised with PLc+Poli Vi (p<0.01). Furthermore, IgG induced in sera of mice immunised with PLc+Poli Vi was similar (p>0.05) to that induced in a group of mice immunised by the parenteral route with the Cuban anti-typhoid vaccine vax-TyVi®, although this vaccine did not induce a mucosal response. In conclusion, this work demonstrates that PLc can be used as a mucosal adjuvant to potentiate the immune response against a polysaccharide antigen like Poli Vi.

Highlights

Enteric infections induced by pathogens are one of the main causes of death all over the world [1]
Our group has been working on the development of a non-living adjuvant/delivery strategy based on proteoliposome derived from Vibrio cholerae O1 (PLc), a proteoliposome-like structure extracted from the strain C7258 of V. cholerae O1 El Tor Ogawa [2]
PLc extraction and characterization PLc structures were obtained from the virulent strain of C7258, V. cholerae O1, El Tor Ogawa using the detergent extraction protocol and observed by TEM (Figure 1A)

Summary

Introduction

Enteric infections induced by pathogens are one of the main causes of death all over the world [1]. Vibrio cholerae kills more than 100 000-130 000 persons each year, most of them children and adolescents between 5 and 19 years old. Current vaccines are mainly based on attenuated or inactivated whole bacterial cells [1]. Our group has been working on the development of a non-living adjuvant/delivery strategy based on PLc, a proteoliposome-like structure extracted from the strain C7258 of V. cholerae O1 El Tor Ogawa [2]. PLc contain important antigens and immunostimulatory molecules like lipopolysaccharides (LPS), OmpU porin and MSHA. Recent work purified Poli Vi vaccines have not been able to prevent Typhoid fever especially in infants [1]

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