Abstract
Zusammenfassung Glomerular basement membranes of normal rats, before and after treatment with collagenase and hyaluronidase, were injected into rabbits. Using the 131I-labelled γ-globulin of each antiserum the amount of kidney-fixing antibody (KFAb) could be determined quantitatively. The hydroxyproline and glucosamine content of glomerular basement membranes was determined after exposure to enzymes. With decollagenized membranes as antigen, the amount of KFAb produced was somewhat diminished. However, immunization with basement membranes, which had been treated with hyaluronidase, resulted in a drastically reduced formation of KFAb. The nature of the apparent effect of hyaluronidase on the antigenic structure of basement membranes is not yet known, especially since there is no concomitant reduction of the glucosamine content. Glomerular basement membranes contain collagen and may function as a weak antigen which fixes anti-kidney antibody as well as cross-reacting KFAb. It is suggested, that the true nephrotoxic antigen (NTA) is not collagen but a glycoprotein participating in the production of KFAb of high affinity for renal antigenic sites.
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