Abstract
AbstractThe mutant p53 gene (mp53; codon 273Arg‐His) was introduced into normal human fibroblasts (OUMS‐24 strain), and a G418‐resistant clone, OUMS‐24/P6, was obtained. This clone expressed mp53 and showed an extended life span, but it senesced at the 79th population‐doubling level (PDL). When these cells were subjected to eight intermittent treatments with 2 Gy of x‐rays, they were immortalized, whereas normal fibroblasts (OUMS‐24) into which mp53 had not been introduced were not immortalized by the same x‐ray treatment. These results indicate that the introduction of mp53 alone is not sufficient for immortalization of human cells and that mutations of the remaining wild‐type p53 or other genes are also necessary. © 1995 Wiley‐Liss, Inc.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
