Immobilized Peptide on the Surface of Poly l-DOPA/Silica for Targeted Delivery of 5-Fluorouracil to Breast Tumor

Abstract

Chemotherapy using drug delivery systems can target tumor cells selectively and do not affect normal cells. In this paper, a specific drug delivery system with immobilized NL2 peptide on the surface of polymeric nano drug was designed for treatment of breast tumor. The tertiary structure of NL2 peptide (AEGEFIHNRYNRFFYWYGDPAK) was selected from the database and synthesized. After that, it was coupled to the synthesized poly 3,4-dihydroxy-l-phenylalanine (DOPA)/SiO2 nano-composite and examined for targeted 5-fluorouracil (5-FU) delivery in nude mice bearing MCF7 human breast carcinoma cells. Investigation of drug deposition in different tissue and in vivo experiments showed drug in combination with functionalized nano-composite with peptide (DPP) improve targeting properties of drug and 5-FU concentration was significantly increased in tumor rather than other tissues. In addition, this functionalization increases antitumor and targeting efficacy than the free 5-FU and non-functionalized nanocomposite.The poly DOPA functionalized peptide is a powerful agent for targeted delivery of anticancer drugs such as 5FU to tumor cells.