Abstract
Lactase has been attached to stainless steel and other dense carriers for use in fluidized bed reactors. The support is activated by coating it with a layer of titanium oxide. Heating the activated support at 538° before contacting with enzyme solution seems to improve catalytic activity and stability of supported lactase. Optimum pH of free and supported enzyme was 4.5. Optimum temperature for free enzyme was 60°; for steel-supported lactase, it was 70°. Heat treated steel-lactase adduct retained 44.5% of initial activity after 5 weeks at 45° under water. The bonding method has also been applied successfully to α-amylase, amyloglucosidase, asparaginase, catalase and trypsin. Inactivated catalyst can be regenerated by heating to 538°, cooling and recontacting with enzyme solution. This is believed to be the first report of enzymes bonded to stainless steel.
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