Abstract
A novel bioaugmentation scheme called immobilized-cell-augmented activated sludge (ICAAS) was developed. Offline enricher reactors were used to maintain immobilized acclimated cells applied to augment completely mixed activated sludge (CMAS) treating a pentachlorophenol (PCP) pulse loading. Cellulose triacetate (CA) and powder activated carbon (PAC) combined with CA (PAC + CA) were the two media types used for entrapping the PCP-degrading culture. With ICAAS at 5% by volume augmentation, PCP removal of 73.1 and 75.1% via biodegradation, volatilization, and adsorption onto suspended cells, entrapped cells, and media was achieved for the systems with CA and PAC + CA media, respectively, while PCP removal in a control CMAS, which had a comparable level of combined PCP adsorption onto suspended cells and volatilization as the ICAAS, was 48.7%. Results further showed that the immobilized cells retained their PCP-degrading ability when they were fed with the inducer (PCP) once every 20 days.
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