Abstract
Lipase from Aspergillus niger was “doubly immobilized” with SiO2 nanoparticles in sol-gel powders prepared via the base-catalyzed polymerization of tetramethoxysilane (TMOS) and methyltreimethoxysilane (MTMS). The hydrolytic activity of the immobilized lipase was measured using the p-nitrophenyl palmitate hydrolysis method. The results showed that the optimum preparation conditions for the gels were made using a MTMS/TMOS molar ratio of 5, 60 mg of SiO2 nanoparticles, a water/silane molar ratio of 12, 120 mg of enzyme supply, and 120 μL of PEG400. Under the optimal conditions, the immobilized lipase retained 92% of the loading protein and 94% of the total enzyme activity. Characteristic tests indicated that the immobilized lipase exhibited much higher thermal and pH stability than its free form, which shows great potential for industrial applications.
Highlights
IntroductionLipases (triacylglycerol acylhydrolase, EC 3.1.1.3) are a group of widely used enzymes distributed in animals, plants, and microorganisms
Lipases are a group of widely used enzymes distributed in animals, plants, and microorganisms
The substrate could not go into the enzyme active site, and the immobilized lipase exhibited low enzyme activity
Summary
Lipases (triacylglycerol acylhydrolase, EC 3.1.1.3) are a group of widely used enzymes distributed in animals, plants, and microorganisms. They can catalyze a variety of reactions, such as esterification, interesterification, and hydrolysis. Aspergillus niger lipase is one of the most well-known lipases [1,2] It displays positional selectivity for the 1- and 3-positions of the glycerol moiety, substrate preference for medium chain length fatty acids, an optimal temperature between 40 ◦ C and 50 ◦ C, an optimal pH from 4 to 7, and is considered GRAS (Generally Regarded As Safe) by the Food and Drug Administration (FDA) of the United States of America [3]. A. niger lipase has been successfully used in many industrial applications, including the food industry, dairy industry, structured lipids synthesis, fat hydrolysis, detergent additive, regioselective deacylation, and the production of biodiesel [5,6,7]
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