Abstract

Abstract In the present work carboxymethylcellulose (CMC) and CMC-gelatin were used as carrier systems for urease immobilization. Immobilization was based on the formation of insoluble salts of CMC and gelatin with chromium(111) ions. Chromium(111) acetate (CA) and chromium(111) sulfate (CS) were used for this purpose and their effect on urease activity was investigated. The activities of immobilized urease using pure and CMC-gelatin carrier systems were compared. Urease activity was determined by using Berthelot method. Reuse number, pH, enzyme and cross linker concentrations and the incubation period were the factors taken into account in this investigation. Activity of immobilized enzymes were found to be stable for at least 2 months and 16–24 usage. Immobilization percentage obtained under optimum conditions was 40%.

Full Text
Paper version not known

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.