Abstract
ABSTRACTIn the present investigation, an collagen fibre (CF), abundant natural biomass, was successfully grafted by polyethyleneimine (PEI). The resultant PEI grafted collagen fibre (CF-PEI) was employed as biocompatible and high cell loading support matrix for the immobilization of Saccharomyces cerevisiae cells. The as-prepared CF-PEI immobilized cells (CF-PEI-cell) exhibited high activity and stability for both batch and continuous fermentation. In batch fermentation, CF-PEI-cells showed enhanced stability as compared with other matrices supported cells, and produced an average ethanol concentration of 45.04 g/L with ethanol yield (YP/S) of 0.46 g/g and glucose conversion efficiency (η) of 90.4%. Continuous fermentation was operated stably in a down-flow trickling bed reactor charged with CF-PEI-cell for a total of 2 months. When the dilution rate was 0.16 1/h, the average ethanol productivity reached 7.18 g/(L h) with η of 88.94%. Further scanning electron microscopy observations confirmed that yeast cells can proliferate on the surface of CF-PEI during ethanol fermentation, which demonstrates that CF-PEI is indeed an ideal matrix for the immobilization of yeast cells.
Highlights
Immobilized cell technology has been extensively applied to ethanol fermentation due to its high cell density, tolerance to higher concentrations of substrate and products, easier separation, etc. [1,2,3]
We systematically investigated the cell viability of immobilized cells and their ethanol fermentation behaviours in batch and continuous fermentation experiments
collagen fibre (CF)-PEI took the lead in cell loading, its specific surface area was not the highest
Summary
Immobilized cell technology has been extensively applied to ethanol fermentation due to its high cell density, tolerance to higher concentrations of substrate and products, easier separation, etc. [1,2,3]. Cell immobilization by adsorption is often preferred for ethanol fermentation because of its simplicity, low cost and high efficiency [4]. The support must be conducive to cell viability [1,2,3] as well as easy to use, cheaper, renewable, biodegradable and available naturally in abundance. Various natural biomass materials have been developed as supports for yeast cell immobilization, including grape skins [5], silk cocoons [6], sugarcane bagasse [7], loofa sponges [8] and bacterial cellulose [9]. The most important advantage accruable from such biomaterials is that their use is free from toxicity problems comparing with inorganic or synthetic support materials
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