Abstract
Porous silicone polymer beads containing viable colonies of Escherichia coli were formed using dimethylsiloxane prepolymer and silicone fluid in a gentle emulsion-polymerization procedure. Viability, cell outgrowth, and amylase production were examined in studies using both batch and continuous cultivation. Significant production of viable cells and amylase was observed. Enhanced plasmid stability through immobilization was demonstrated when cells were grown in M9/glycerol medium without antibiotic.
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