Immobilization of Purified RNase of Bacillus sp. RB-3 with Moringa Gum Polyvinyl alcohol (MGP) hydrogel and observing its effects on the Hep-2C cell lines

Abstract

RNase is a type of nuclease that catalyzes the degradation of RNA into smaller components. RNase are found greatly in living organisms and are known to be involved in gene expression regulation and RNA metabolism. Present study was attempted for the purification and the applications of the bacterial RNase. Purified RNase showed the presence of a single band of 28 kDa on SDS-PAGE. FTIR spectral studies successfully confirmed the synthesis of MGP and immobilization of RNase onto MGP. The optimum substrate, temperature, time and pH for free and hydrogel-bound RNase recovered from Bacillus sp. RB-3 were found 150μg/mL for hydrogel bound, 200 μg /mL for free RNase, 37°C, 30 (min) and 7.4 respectively. Moreover, RNase is cytotoxic against Hep-2C cell lines.