Abstract
We demonstrated developments of a non-labeling DNA (deoxyribonucleic acid) sensor by using a commercially available ion-sensitive field-effect transistor (IS-FET). A single strand DNA was immobilized on a Ta2O5 thin film on a gate electrode in the IS-FET, and the surface of the thin film was confirmed by XPS measurements. The results indicated that the DNA was immobilized on the Ta2O5 thin film. To detect the hybridization of DNA, we carried out measurements of Idrain-Vgs properties of IS-FETs immobilized by ss-DNA and ds-DNA hybridized by complemental target DNA. Then, the difference in threshold voltage was evaluated to be about 10 mV, and the amount of immobilized DNA as 6.3×107 [molecules/cm2]. Consequently, the non-labeling IS-FET DNA sensor is expected to be a novel measurement tool for diagnosing diseases.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
