Immobilization of porcine pancreatic lipase onto ionic liquid modified mesoporous silica SBA-15

Abstract

Ionic liquid was synthesized and grafted onto the surface hydroxyl of mesoporous silica SBA-15 (IL–SBA). Porcine pancreas lipase (PPL) was successfully incorporated into the ionic liquid modified SBA-15 material by physical adsorption (PPL–IL–SBA). The enzymatic properties, including optimum temperature, pH and reusability of PPL–IL–SBA, were investigated in the triacetin hydrolysis reaction. Compared with parent SBA-15 immobilized lipase (PPL–SBA), the enzymatic properties have dramatically improved, especially the optimized activity increased from 594 to 975 U/g PPL after modification.