Immobilization of porcine pancreatic lipase in zeolite MCM 22 with different Si/Al ratios

Cléber Calgaroto,Robison P Scherer,Selma Calgaroto,J Vladimir Oliveira,Débora De Oliveira,Sibele B.C Pergher

doi:10.1016/j.apcata.2010.12.032

Abstract

The use of zeolites as support for immobilization of enzymes has been a matter of great interest recently due to the potential properties of these materials. In this work, the MCM 22 zeolite with different Si/Al ratios (15, 25 and 50) was used as support for immobilization of porcine pancreatic lipase, assessing the effect of its structure on the immobilization yield and enzyme activity. Results showed that the material composition influenced significantly the immobilization process. Higher yields of immobilization and enzymatic activities were achieved when MCM 22 with Si/Al ratio of 25 was used as support. In general, results demonstrate the potential of MCM 22 as support for porcine pancreatic lipase immobilization.

Highlights

The use of zeolites as support for enzymes immobilization has being a subject of growing interest due to the potential properties of this class of materials [1,2,3,4,5,6]
Zeolites are microporous materials that present an important role in several technological areas [7,8], mainly due to their high specific area, adsorption ability and presence of active acid centers [9]
The zeolite MCM 22 has a precursor of lamellar structure, constituted by lamellas of 25 Aof thickness with only one system of pores formed by sinusoidal channels, constituted by opening rings of 10 members (10 MR)

Summary

Introduction

The use of zeolites as support for enzymes immobilization has being a subject of growing interest due to the potential properties of this class of materials [1,2,3,4,5,6]. Zeolites are microporous materials that present an important role in several technological areas [7,8], mainly due to their high specific area, adsorption ability and presence of active acid centers [9]. These compounds are crystalline aluminosilicates with a structure based on the tridimensional combination of tetrahedrons TO4 (T = Si, Al), linked by oxygen atoms. The aluminosilicate MCM 22 presents high specific area, thermal stability and acidity, desirable characteristics for use as support for immobilization of biomolecules

Objectives

Methods

Conclusion