Immobilization of poly(lactide-co-glycolide) microspheres on bone implant materials for antibiotic release and the binding mechanisms.

Abstract

Bone implants are susceptible to postoperative infections. Immobilization of antibiotic-loaded microparticles on implants is an effective approach to addressing this problem. Immobilization methods reported in earlier studies frequently used special or potentially harmful conditions. Therefore, the present study explored a new method to immobilize poly(lactide-co-glycolide) (PLGA) microspheres on bone implant materials. PLGA microspheres were prepared by an emulsion method using polyvinyl alcohol (PVA) as an emulsifier. The microspheres were immobilized on two commonly used orthopaedic biomaterials [hydroxyapatite-coated titanium (HA-Ti) and poly(methyl methacrylate) (PMMA)] by dispersing on the surface followed by vacuum drying. Microspheres were retained stably on both materials even after immersion in phosphate-buffered saline for 12 d. Pretreatment of microspheres with sodium borate (i.e., an eliminator of hydroxyl groups of PVA) substantially reduced their retention on HA-Ti, but only moderately reduced their retention on PMMA. This suggested that the binding of the residual PVA on the microspheres to the HA coating is the dominant contributor to their immobilization on HA-Ti, whereas other forces contributed substantially to their immobilization on PMMA. Microspheres containing ciprofloxacin (a water-soluble antibiotic) and triclosan (an oil-soluble antibiotic) were immobilized on HA-Ti and PMMA, respectively. They effectively killed adjacent bacteria. These results offer a simple and versatile method for immobilizing drug-release microspheres on some important bone implant surfaces.