Abstract
Surface modified Multi-walled carbon nanotubes (MWCNTs) Buckypaper/Polyvinyl Alcohol (BP/PVA) composite membrane was synthesized and utilized as support material for immobilization of Jicama peroxidase (JP). JP was successfully immobilized on the BP/PVA membrane via covalent bonding by using glutaraldehyde. The immobilization efficiency was optimized using response surface methodology (RSM) with the face-centered central composite design (FCCCD) model. The optimum enzyme immobilization efficiency was achieved at pH 6, with initial enzyme loading of 0.13 U/mL and immobilization time of 130 min. The results of BP/PVA membrane showed excellent performance in immobilization of JP with high enzyme loading of 217 mg/g and immobilization efficiency of 81.74%. The immobilized system exhibited significantly improved operational stability under various parameters, such as pH, temperature, thermal and storage stabilities when compared with free enzyme. The effective binding of peroxidase on the surface of the BP/PVA membrane was evaluated and confirmed by Field emission scanning electron microscopy (FESEM) coupled with Energy Dispersive X-Ray Spectroscopy (EDX), Fourier transform infrared spectroscopy (FTIR) and Thermogravimetric Analysis (TGA). This work reports the characterization results and performances of the surface modified BP/PVA membrane for peroxidase immobilization. The superior properties of JP-immobilized BP/PVA membrane make it promising new-generation nanomaterials for industrial applications.
Highlights
Selection of appropriate immobilization method, support materials, and operating conditions for enzyme immobilization plays a critical role in achieving superior enzyme performances and stabilization
Characterization studies of Jicama peroxidase (JP)-immobilized buckypaper/polyvinyl alcohol (BP/PVA) membrane were performed using as Field emission scanning electron microscopy (FESEM), Energy Dispersive X-Ray Spectroscopy (EDX), Fourier transform infrared spectroscopy (FTIR), and Thermogravimetric Analysis (TGA) to facilitate in evaluating the biocompatibility of the peroxidases and BP/PVA membrane
Similar results were reported by Chiong, et al.[34], where 1.22 U/mL JP was extracted from the jicama skin peels
Summary
Selection of appropriate immobilization method, support materials, and operating conditions for enzyme immobilization plays a critical role in achieving superior enzyme performances and stabilization. Despite the superior properties of nano-structured support materials for enzyme immobilization technologies, it is impractical to use nano-scale materials for scaling-up and industrial continuous operating systems[24] Their extremely small sizes resulted in difficult for separation from the reaction medium for further recovery and reusability purposes. PVA was chosen as the reinforcement polymer since the hydroxyl groups in PVA can form strong hydrogen bonding with the hydrophilic surface of f-MWCNTs. Besides, previous studies show BP/PVA membrane as a promising next-generation nanocomposite material due to its robust thermal, mechanical, electrical and electromechanical properties[25,26]. The main objective is to study the immobilization of peroxidase extracted from jicama skin peels on functionalized BP/PVA membrane via covalent bonding Another aim is to develop a robust and reliable optimization model for enzyme immobilization efficiency on BP/PVA membrane. This study provides an insight on the mechanism of enzyme immobilization on a nano-structured support material
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