Immobilization of Pectinolytic Enzymes on Nylon 6/6 Carriers

Sana Ben-Othman,Toonika Rinken

doi:10.3390/app11104591

Sana Ben-Othman, Toonika Rinken

Open Access

https://doi.org/10.3390/app11104591

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Abstract

Pectinolytic enzymes are an important tool for sustainable food production, with a wide range of applications in food processing technologies as well as the extraction of bioactive compounds from pectin-rich raw materials. In the present study, we immobilized commercial pectinase preparation onto pellet and thread shaped nylon 6/6 carriers and assessed its stability and reusability. Five commercial pectinase preparations were tested for different pectin de-polymerizing activities (pectinase, polygalacturonase, and pectin lyase activities). Thereafter, Pectinex® Ultra Tropical preparation, exhibiting the highest catalytic activities among the studied preparations (p < 0.0001), was immobilized on nylon 6/6 using dimethyl sulfate and glutaraldehyde. The immobilization yield was in accordance with the carrier surface area available for enzyme attachment, and it was 1.25 ± 0.10 U/g on threads, which was over 40 times higher than that on pellets. However, the inactivation of immobilized enzymes was not dependent on the shape of the carrier, indicating that the attachment of the enzymes on the surface of nylon 6/6 carriers was similar. The half-life of enzyme inactivation fast phase at 4 °C was 12.8 days. After 5 weeks, the unused threads retained 63% of their initial activity. Reusability study showed that after 20 successive cycles the remaining activity of the immobilized pectinase was 22%, indicating the good prospects of reusability of the immobilized enzyme preparations for industrial application.

Highlights

Introduction and Maria GulloPectinolytic enzymes, known as pectinases, are a heterogeneous group of enzymes catalyzing different steps of the hydrolysis of pectin, a major component of plant cell walls [1]
We examined the effect of dimethyl sulfate (DMS) treatment duration (1–7.5 min) on pectinase immobilization, as in addition to generating active groups, the mechanical strength of nylon 6/6 can decrease after a longer treatment over 5 min
Commercial preparation was results immobilized onto pectinase immobilization yield per g of carrier was more than times higher in nylon

Summary

Introduction

Pectinolytic enzymes, known as pectinases, are a heterogeneous group of enzymes catalyzing different steps of the hydrolysis of pectin, a major component of plant cell walls [1]. Based on their mode of action, pectinases are classified as (i) de-esterifying enzymes (pectin esterase), and (ii) de-polymerizing enzymes (hydrolases, lyases). Commercial pectinase preparations are widely utilized in the extraction and clarification of fruits and vegetables juices [6] They are used for the extraction of vegetable oil [7] and the fermentation of coffee beans and tea leaves [2]. Along with cellulases, pectinases are an important tool for ‘green’

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