Abstract
Whole cells from Paracoccus denitrificans IFO 12442 were immobilized with a polyelectrolyte complex composed of potassium poly(vinyl alcohol) sulfate (KPVS) and poly(diallyldimethylammonium chloride) (PDDA) by the following procedures: An excess of PDDA was first mixed with a cell suspension to aggregate cells, then KPVS was added to form a complex with excess PDDA and to entrap the aggregated cells. Electron microscopic analysis showed that the aggregated cells were entrapped or surrounded by an amorphous complex support. The rate of nitrate reduction or carbon consumption by the immobilized cells was almost the same as that by the free cells, as determined by anaerobic incubation using a non-growth medium containing KNO 3 as a substrate and potassium aspartate as a carbon source. The immobilized cells exhibited activity at pH 4, at which the free cells lost their activity. The initial activity of the immobilized cells remained stable for at least one month in a phosphate buffer with gentle stirring.
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