Abstract
Ionic liquid( IL) 1-butyl-3-methylimidazolium chloride( [Bmim]Cl) was used to dissolve microcrystalline cellulose Avicel to make IL-regenerated cellulose membrane for papain immobilization through silane modification method. It demonstrated that the immobilized papain could be obtained at the condition of6. 5%( mass fraction) Avicel /[BMIM]Cl for membrane as the solid support; 30% NH_2( CH_2)_3Si( OC_2H_5)_3at 50 ℃ to functionalize the carrier; adsorbing for 3 h followed by cross-linking for 4 h at 50 ℃,p H = 7. 0 and enzyme concentration of 300 mg/m L,using 10%( volume fraction) glutaraldehyde as the cross-linker. After immobilization,papain was immobilized to the both sides of the regenerated cellulose membrane surfaces with high loading density. The immobilized papain could be limited on the wool fiber surface,only digesting the scale structure instead of native papain penetrating inside the fiber and generating the significant weight and strengthen loss.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
