Abstract
As an important enzyme, xylanase is widely used in the food, pulp, and textile industry. Different applications of xylanase warrant specific conditions including temperature and pH. This study aimed to carry out sodium alginate beads as carrier to immobilize previous reported mutated xylanase from Neocallimastix patriciarum which expressed in E. coli, the activity of immobilization of mutated xylanase was elevated about 4% at pH 6 and 13% at 62 °C. Moreover, the immobilized mutated xylanase retained a greater proportion of its activity than the wide type in thermostability. These properties suggested that the immobilization of mutated xylanase has potential to apply in biobleaching industry.
Highlights
Xylan, a component of hemicelluloses which are the second most abundant plant fraction available in nature, accounts for as much as 30% of the dry weight of some plant tissues (Ebringerová and Heinze, 2000)
More studies indicate microbial xylanases can be widely applied in the biobleaching industry, food and beverage industries and animal feeding industry (Atalah et al, 2019; Campioni et al, 2019)
Sodium alginate beads is relatively big in size, eco-friendly, low cost, high loading capacity and easy prepared are widely used in immobilization study (Cheng et al, 2016)
Summary
A component of hemicelluloses which are the second most abundant plant fraction available in nature, accounts for as much as 30% of the dry weight of some plant tissues (Ebringerová and Heinze, 2000). Biodegradation of xylan requires the action of several enzymes, among which xylanases (1,4-β-D xylan xylanohydrolas, EC 3.2.1.8) play a key role (Biely et al, 1997). Due to the important industrial application, many xylanase genes have been identified in bacteria and fungi (Chadha et al, 2019). Xylanases are grouped into glycosidase families based on the primary structure of the catalytic domains and are normally reported in glycosidase families 10 and 11 (Jeffries, 1996; Collins et al, 2005). Xylanase still has some obstacles in the application of pulp bioleaching industries, which makes it unable to be widely used in this field. Free xylanase is often hampered by its reusability and limited by high production costs, low enzyme activity and high consumption of xylanase. Immobilized enzymes were allowed the enzyme to be reused in multiple cycles to lower the production costs and overcome such technical drawbacks (Pal and Khanum, 2011; Zhao et al, 2019)
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