Immobilization of multi-enzyme in porous solid supports—a theoretical study

Abstract

A theoretical study is presented dealing with the immobilization of two enzymes into a porous solid support. The developed model basically takes into account the interplay between restricted diffusions of two enzymes within intraparticle void space and their competitive adsorptions onto available functional sites on the interior surfaces. Simulations have shown that parameters such as binding rates of enzymes, initial bulk concentration ratio, pellet size and impregnating time have significant influence on the eventual distribution of two enzymes within the porous solid supports.