Immobilization of Multi-Enzymes on Support Materials for Efficient Biocatalysis.

Kongliang Xu,Renchao Zheng,Xuexiao Chen,Yuguo Zheng

doi:10.3389/fbioe.2020.00660

Kongliang Xu, Renchao Zheng + Show 2 more

Open Access

https://doi.org/10.3389/fbioe.2020.00660

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Abstract

Multi-enzyme biocatalysis is an important technology to produce many valuable chemicals in the industry. Different strategies for the construction of multi-enzyme systems have been reported. In particular, immobilization of multi-enzymes on the support materials has been proved to be one of the most efficient approaches, which can increase the enzymatic activity via substrate channeling and improve the stability and reusability of enzymes. A general overview of the characteristics of support materials and their corresponding attachment techniques used for multi-enzyme immobilization will be provided here. This review will focus on the materials-based techniques for multi-enzyme immobilization, which aims to present the recent advances and future prospects in the area of multi-enzyme biocatalysis based on support immobilization.

Highlights

In nature, almost all the cascade reactions in cell are catalyzed by the cooperation of various enzymes (Ricca et al, 2011; Sheldon and Woodley, 2018; Shi et al, 2018)
Enzymes will be close to one another and the mass transfer limitation can be reduced through coimmobilization, which has been proved to increase the enzymatic activity via substrate channeling and improve the stability and reusability
As support materials can strongly affect the properties of enzymes, support selection has been considered as a hot topic in the field of enzyme immobilization

Summary

Introduction

Almost all the cascade reactions in cell are catalyzed by the cooperation of various enzymes (Ricca et al, 2011; Sheldon and Woodley, 2018; Shi et al, 2018). First case of co-immobilization of GOx and glucoamylase onto chemically reduced graphene oxide via non-covalent bonds for one-pot production of gluconic acid from starch (Zhao et al, 2014). CNTs have received growing attention for multi-enzyme immobilization (Table 2), due to their high specific surface area, large aspect ratios, excellent chemical stability, etc.

Results

Conclusion