Abstract
Inefficient sewage management has caused contamination of our water resources (rivers and lakes) that inspires researchers to constantly innovate and upgrade the existing wastewater treatment technologies. This study explores the possibility of a microbial consortium immobilized on different natural matrix for sewage treatment at lab-scale bioreactor. Bacterial isolates having lipolytic, proteolytic, cellulolytic and amylolytic activities were included in consortia as these are the major constitutes of sewage. The activities of these bacterial cultures were confirmed using different microbiological and molecular tools. The microbial consortium immobilized on natural coconut coir was found to be the most effective matrix to enhance the efficiency of sewage treatment. The immobilization of microbes on a natural dry coconut coir matrix was confirmed through scanning electron microscopy (SEM). Two Lab-scale aerobic bioreactors were setup in parallel for the treatment of sewage and simulated wastewaters. Both the reactors were operated in continuous mode for 15 and 16 days and efficiency of treatment was analysed by monitoring chemical oxygen demand (COD) removal, nitrogen removal as NH4-N and NO3-N. Simultaneously, the nitrogen removal activity was validated in all members of microbial consortium using gene-specific primers. Results show that 75.9% and 73.7% COD removal were observed in column C and D and nitrogen as NO3-N 78.9% and 79.7% (NH4-N) removal were measured in the effluent of reactors. Similarly, 93% COD, 78.1% NO3-N and 79.7% NH4-N removal were observed in a stimulated wastewater reactor. This significant removal of organic material shows the higher efficiency of the reactor which could be used for field application for sewage treatment.
Published Version
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