Abstract
The aim of the present investigation is dealing with the role of different cellulosic materials in stabilization of L-methionine γ-lyase (MGL) using physical adsorbed immobilization green protocol for volatile sulfur compounds (VSCs) production. Immobilization efficiency and enzyme activity of MGL were evaluated for each of tested cellulosic material. In comparison, green cellulose nanofibers (GCNFs) were found to be promising nano-materials followed by microcrystalline cellulose (MCC) in terms of immobilization effiiciency with 60.2 % and 43% respectively. Enzyme assays demonstrated that the immobilized enzyme exhibited better thermal stability than the original one. Comparative evaluation of the free and immobilized enzyme proved that, the GCNFs-MGL was more acidic pH-stability and more halo-tolerant than the free one. In consequence of these properties, the immobilized enzyme was able to produce of VSCs via L-methionine hydrolyze more extensively under the cheese ripening conditions. Successfully immobilization of the Streptomyces DMMMH60 MGL on the GCNFs by a very simple adsorption protocol, offering an efficient and cheap method for performing repeated enzymatic hydrolysis reactions.
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