Immobilization of immunoglobulin on a quartz surface by BrCN without loss of antigen binding capability

Abstract

BrCN-activated quartz was used for the immobilization of monoclonal immunoglobulins. BrCN-activated quartz keeps its ability to quantitatively bind immunoglobulins for at least 8 h. The surface density of the immobilized immunoglobulins was 100 ng cm −2 and they were found to retain their ability to bind antigen in a molar ratio of 1:2 for not less than three months.