Abstract
In this investigation, mesoporous TiO2-SiO2 composites with different Ti/Si molecular ratios were synthesized by one pot method. Apart from the characterization by small angle X-ray diffraction (SAXRD), transmission electron microscope (TEM) and N2 absorption-desorption isotherms, these mesoporous materials were used in the adsorption and enrich of biological molecules. The results demonstrate that TiO2-SiO2 composites possess the ordered mesoporous structure with uniform pore. Bovine hemoglobin (BHb) can be immobilized within the mesopore of the composite and the immobilization amount increases with the Ti/Si molecular ratio. It is speculated that the strong electrostatic interaction between biomolecule and the inner surface of mesoporous materials enhances the adsorption of BHb. Moreover, our investigation confirms that the absorbed BHb still effectively retains its bioactivity, which is very significant for the potential application of this hybrid material in biocatalysis.
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