Abstract
Plasma induced graft copolymerization of acrylic acid, which was incorporated onto PE films, was investigated here. Glucose oxidase was immobilized onto this novel grafted polymeric film with and without using poly(ethylene oxide) used as spacer. ESCA was used for analyzing the plasma treated PE, plasma induced polyacrylic acid graft PE and poly(ethylene oxide) modified PAAc graft PE films. The immobilized GOD with spacer (PAAc-PEO-GOD) was observed to record a higher activity than that without spacer (PAAc-GOD). The pH and thermal stabilities of the immobilized GOD without spacer (PAAc-GOD) were higher than those of the immobilized GOD with spacer (PAAc-PEO-GOD) as well as free enzyme.
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