Abstract
The effect of aeration on the stability of glucose oxidase in the reaction of glucose oxidation to gluconic acid was investigated by determining the operational stability decay rate constant in the process conditions. Eupergit C as a porous carrier was chosen for the enzyme immobilization. To evaluate glucose oxidase operational stability during process conditions, experiments of glucose oxidation were carried out in the repetitive batch reactor with and without continuous aeration at different aeration levels. It was found that the decay rate of the free enzyme linearly depended on the air flow rate. Immobilization of glucose oxidase on Eupergit C significantly enhanced enzyme stability at higher aeration rates. Kinetics of the free and immobilized enzyme was also determined. The mathematical model of glucose oxidation catalysed by free and immobilized glucose oxidase in the batch reactor was developed.
Highlights
Enzymatic oxidation is an important reaction for production of valuable products[1]
Eupergit C is porous carrier, and during the immobilization process, approximately 75–80 % enzyme solution soaked onto the carrier, and the mass of the carrier increased significantly
The results showed that the immobilization of the catalase was slightly better than the immobilization of Glucose oxidase (GOD)
Summary
Enzymatic oxidation is an important reaction for production of valuable products[1]. The advantages over chemical oxidation are high selectivity and low environmental impact, since enzymes are active under mild conditions[2]. Biocatalytic oxidations use oxygen as the electron acceptor, which is available and inexpensive. The limitation considering oxygen utilisation is its slow mass transfer from air to aqueous phase. In order to overcome this problem, a continuous supply of oxygen in the reactor is necessary to achieve reasonable reaction rates. The method for oxygen delivery usually causes irreversible enzyme inactivation, which is commonly attributed to the hydrodynamic shear stress and gas-liquid interface[2]
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