Immobilization of gelatin onto acrylic acid grafted bacterial cellulose for regulating cell behaviors using gamma-irradiation

Abstract

Event Abstract Back to Event Immobilization of gelatin onto acrylic acid grafted bacterial cellulose for regulating cell behaviors using gamma-irradiation Youn-Mook Lim1, Jin-Oh Jeong1*, Sung-Jun Ahn1*, Sung In Jeong1*, Hui-Jeong Gwon1, Jong-Seok Park1 and Jae Young Lee2 1 Korea Atomic Energy Research Institute, Research Division for Industry & Environment, Korea 2 Gwangju Institute of Science and Technology, School of Materials Science & Engineering, Korea Bacterial cellulose(BC) scaffolds are generated from gluconacetobacter hansenii TL-2C. BC has been shown to have a high-burst pressure and ultrafine highly nanofibrous structure similar with that in a natural extracellular matrix (ECM) for tissue engineering. In this study, acrylic acid(AAc) was grafted onto BC surface using gamma-irradiation and then gelatin immobilized it using EDC/NHS reaction. The characterization of the scaffolds was performed by scanning electron microscopy, ATR-FTIR spectroscopy, a toluidine blue O assay, and 2,4,6,-trinitro-benzensulfonic acid assay. The surface morphology of scaffolds was confirmed BC membranes by SEM for gelatin and AAc grafted it, so that it did not change surface morphology. The adhesion and spreading of human mesenchymal stem cells was improved on the gelatin-AAc-BC nanofiber compared to unmodified BC and AAc-BC nanofiber. Therefore, gelatin and AAc grafted BC scaffolds have been possible to tissue scaffold that accelerate the cell behavior for biomedical applications. Keywords: Tissue Engineering, biomaterial, Biocompatibility, 3D scaffold Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Biomimetic materials Citation: Lim Y, Jeong J, Ahn S, Jeong S, Gwon H, Park J and Lee J (2016). Immobilization of gelatin onto acrylic acid grafted bacterial cellulose for regulating cell behaviors using gamma-irradiation. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.01743 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. * Correspondence: Dr. Jin-Oh Jeong, Korea Atomic Energy Research Institute, Research Division for Industry & Environment, Jeongeup-si, Jeollabuk-do, Korea, nanjinoh@gmail.com Dr. Sung-Jun Ahn, Korea Atomic Energy Research Institute, Research Division for Industry & Environment, Jeongeup-si, Jeollabuk-do, Korea, Email1 Dr. Sung In Jeong, Korea Atomic Energy Research Institute, Research Division for Industry & Environment, Jeongeup-si, Jeollabuk-do, Korea, Email2 Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Youn-Mook Lim Jin-Oh Jeong Sung-Jun Ahn Sung In Jeong Hui-Jeong Gwon Jong-Seok Park Jae Young Lee Google Youn-Mook Lim Jin-Oh Jeong Sung-Jun Ahn Sung In Jeong Hui-Jeong Gwon Jong-Seok Park Jae Young Lee Google Scholar Youn-Mook Lim Jin-Oh Jeong Sung-Jun Ahn Sung In Jeong Hui-Jeong Gwon Jong-Seok Park Jae Young Lee PubMed Youn-Mook Lim Jin-Oh Jeong Sung-Jun Ahn Sung In Jeong Hui-Jeong Gwon Jong-Seok Park Jae Young Lee Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.